Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

DAXX Antikörper (AA 56-345)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DAXX in WB, IHC, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.
Produktnummer ABIN3043822

Kurzübersicht für DAXX Antikörper (AA 56-345) (ABIN3043822)

Target

Alle DAXX Antikörper anzeigen
DAXX (Death-Domain Associated Protein (DAXX))

Reaktivität

  • 159
  • 61
  • 50
  • 7
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte, Maus

Wirt

  • 142
  • 18
Kaninchen

Klonalität

  • 131
  • 29
Polyklonal

Konjugat

  • 62
  • 7
  • 7
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
Dieser DAXX Antikörper ist unkonjugiert

Applikation

  • 131
  • 65
  • 65
  • 38
  • 36
  • 24
  • 21
  • 19
  • 16
  • 14
  • 7
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 23
    • 21
    • 16
    • 16
    • 15
    • 9
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 56-345

    Verwendungszweck

    Anti-Daxx Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Daxx Antibody Picoband® (ABIN3043822). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human Daxx recombinant protein (Position: K56-R345). Human Daxx shares 88.3% and 88.6% amino acid (aa) sequence identity with mouse and rat Daxx, respectively.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
    Immunocytochemistry , 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. Chang, H. Y., Nishitoh, H., Yang, X., Ichijo, H., Baltimore, D. Activation of apoptosis signal-regulating kinase 1 (ASK1) by the adapter protein Daxx. Science 281: 1860-1863, 1998. 2. Kiriakidou, M., Driscoll, D. A., Lopez-Guisa, J. M., Strauss, J. F., III. Cloning and expression of primate Daxx cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region. DNA Cell Biol. 16: 1289-1298, 1997. 3. Yang, X., Khosravi-Far, R., Chang, H. Y., Baltimore, D. Daxx, a novel Fas-binding protein that activates JNK and apoptosis. Cell 89: 1067-1076, 1997.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    DAXX (Death-Domain Associated Protein (DAXX))

    Andere Bezeichnung

    DAXX

    Hintergrund

    Synonyms: Death domain-associated protein 6,Daxx,hDaxx,ETS1-associated protein 1,EAP1,Fas death domain-associated protein,DAXX,BING2, DAP6,

    Tissue Specificity: Ubiquitous.

    Background: DAXX (death-domain associated protein) also known as DAP6 (Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. Human DAXX encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses by Yang et al. (1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.

    Sequence Similarities: Belongs to the DAXX family.

    Molekulargewicht

    115 kDa

    Gen-ID

    1616

    Pathways

    Intracellular Steroid Hormone Receptor Signaling Pathway
Sie sind hier:
Chat with us!