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CTCF Antikörper (AA 521-727)

CTCF Reaktivität: Human, Maus, Ratte WB, IF, ICC, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3043817
  • Target Alle CTCF Antikörper anzeigen
    CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))
    Bindungsspezifität
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 521-727
    Reaktivität
    • 48
    • 31
    • 27
    • 7
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    Human, Maus, Ratte
    Wirt
    • 40
    • 7
    • 2
    • 1
    • 1
    Kaninchen
    Klonalität
    • 29
    • 22
    Polyklonal
    Konjugat
    • 37
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser CTCF Antikörper ist unkonjugiert
    Applikation
    • 31
    • 12
    • 11
    • 11
    • 9
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Verwendungszweck
    Anti-CTCF Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-CTCF Antibody Picoband® (ABIN3043817). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human CTCF recombinant protein (Position: K521-R727). Human CTCF shares 94% and 93.1% amino acid (aa) sequence identity with mouse and rat CTCF, respectively.
    Isotyp
    IgG
    Top Product
    Discover our top product CTCF Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
    1. Chaumeil J, Skok JA (April 2012). "The role of CTCF in regulating V(D)J recombination". Curr. Opin. Immunol. 24(2): 153-9. 2. Filippova GN, Fagerlie S, Klenova EM, Myers C, Dehner Y, Goodwin G, Neiman PE, Collins SJ, Lobanenkov VV (June 1996). "An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes". Mol. Cell. Biol. 16 (6): 2802-13. 3. Phillips JE, Corces VG (June 2009)."CTCF: master weaver of the genome".Cell 137 (7): 1194-211.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))
    Andere Bezeichnung
    CTCF (CTCF Produkte)
    Hintergrund

    Synonyms: Transcriptional repressor CTCF,11-zinc finger protein,CCCTC-binding factor,CTCFL paralog,CTCF,

    Tissue Specificity: Ubiquitous. Absent in primary spermatocytes. .

    Background: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. And this nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

    Sequence Similarities: Belongs to the CTCF zinc-finger protein family.

    Molekulargewicht
    150 kDa
    Gen-ID
    10664
    UniProt
    P49711
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