ATP2A1/SERCA1 Antikörper (N-Term)

Produktnummer ABIN3043792

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ATP2A1/SERCA1 in WB, IHC und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für ATP2A1/SERCA1 Antikörper (N-Term) (ABIN3043792)

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP2A1/SERCA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-ATP2A1/SERCA1 Antikörper

Bindungsspezifität: AA 1-32, N-Term

Verwendungszweck: Anti-SERCA1 ATPase/ATP2A1 Antibody

Sequenz: MEAAHAKTTE ECLAYFGVSE TTGLTPDQVK RN

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-SERCA1 ATPase/ATP2A1 Antibody (ABIN3043792). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SERCA1 ATPase, different from the related mouse and rat sequences by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ATP2A1/SERCA1

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Andere Bezeichnung: ATP2A1

Hintergrund:

Synonyms: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1,SERCA1,SR Ca (2+)-ATPase 1,3.6.3.8,Calcium pump 1,Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform,Endoplasmic reticulum class 1/2 Ca (2+) ATPase,ATP2A1,

Tissue Specificity: Skeletal muscle, fast twitch muscle (type II) fibers.

Background: SERCA1, also called ATP2A1, is an enzyme that in humans is encoded by the ATP2A1 gene. This gene encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. The SERCA1 gene is mapped to 16p11.2. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. It has been determined that the human SERCA1 gene is 26 kb long and contains 23 exons, of which can be alternatively spliced. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise.

Molekulargewicht: 110 kDa

Gen-ID: 487

UniProt: O14983

Pathways: Ribonucleoside Biosynthetic Process