Nerve Growth Factor Antikörper (N-Term)

Produktnummer ABIN3043754

Produktdetails anti-Nerve Growth Factor Antikörper

Target: Nerve Growth Factor (NGF)

Bindungsspezifität: AA 162-173, N-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nerve Growth Factor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Anti-Beta-nerve growth factor NGF Antibody Picoband®

Sequenz: EVNINNSVFK QY

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Beta-nerve growth factor NGF Antibody (ABIN3043754). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NGF, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Dracopoli, N. C., Rose, E., Whitfield, G. K., Guidon, P. T., Bale, S. J., Chance, P. A., Kourides, I. A., Housman, D. E. : Two thyroid hormone regulated genes, the beta-subunits of nerve growth factor (NGFB) and thyroid stimulating hormone (TSHB), are located less than 310 kb apart in both human and mouse genomes. Genomics 3: 161-167, 1988. 2. Francke, U., de Martinville, B., Coussens, L., Ullrich, A. : The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. Science 222: 1248-1251, 1983 3. Einarsdottir, E., Carlsson, A., Minde, J., Toolanen, G., Svensson, O., Solders, G., Holmgren, G., Holmberg, D., Holmberg, M. : A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception. Hum. Molec. Genet. 13: 799-805, 2004.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Nerve Growth Factor Antikörper (ABIN3043754)

Fan, Xiong, Fu, Xu, Wang, Chen, Xia, Peng, Ye, Wang, Zhang, Ye et al.: "Polyaniline promotes peripheral nerve regeneration by enhancement of the brain‑derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling ..." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 7534-7540, (2018) (PubMed).

Luo, Liu, Chen, Wang, Zeng, Chen, Cheng et al.: "Nerve growth factor and inducible nitric oxide synthase expression in the mesencephalon and diencephalon, as well as visual- and auditory-related nervous tissues, in a macaque model of type 2 ..." in: Neural regeneration research, Vol. 7, Issue 4, pp. 302-7, (2015) (PubMed).

Zhao, Chen, Shen, Zhao, Wang, Shi, Wang, Cui, Yan, Xue: "Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury." in: Neural regeneration research, Vol. 7, Issue 10, pp. 741-8, (2015) (PubMed).

Lu, Liu, Zhu, Xu: "Expression of nerve growth factor and hypoxia inducible factor-1? and its correlation with angiogenesis in non-small cell lung cancer." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 3, pp. 359-62, (2014) (PubMed).

Li, Ma, Yi, Wang, Li, Liu, Sun, Chen, Yu, Li, Chen, Zhou: "The interactions between nerve growth factor and gonadotrophins in bovine oviduct." in: Animal reproduction science, Vol. 149, Issue 3-4, pp. 117-23, (2014) (PubMed).

Huang, Zhang, Xu, Li, Zhao: "Expression of nerve growth factor in the prostate of male rats in response to chronic stress and sympathetic denervation." in: Experimental and therapeutic medicine, Vol. 8, Issue 4, pp. 1237-1240, (2014) (PubMed).

Zhao, Ding, Guo, Chen: "Intrathecal lidocaine neurotoxicity: combination with bupivacaine and ropivacaine and effect of nerve growth factor." in: Life sciences, Vol. 112, Issue 1-2, pp. 10-21, (2014) (PubMed).

Li, Shu, Tang, Deng, Fu, Lv: "?-Nerve growth factor attenuates hepatocyte injury induced by D-galactosamine in vitro via TrkA NGFR." in: Molecular medicine reports, Vol. 8, Issue 3, pp. 813-7, (2013) (PubMed).

Pu, Shi, Wang, Wang, Guo, Liu, Sun, Gao, Zhou: "Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats." in: Journal of biomedical research, Vol. 25, Issue 4, pp. 292-8, (2013) (PubMed).

Sun, Guo, Zheng, Zhang, Li, Liu, Yin, Yang, Shu: "Increased expression of TRPV1 in the cortex and hippocampus from patients with mesial temporal lobe epilepsy." in: Journal of molecular neuroscience : MN, Vol. 49, Issue 1, pp. 182-93, (2012) (PubMed).

Orita, Koshi, Mitsuka, Miyagi, Inoue, Arai, Ishikawa, Hanaoka, Yamashita, Yamashita, Eguchi, Toyone, Takahashi, Ohtori: "Associations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the knee." in: BMC musculoskeletal disorders, Vol. 12, pp. 144, (2012) (PubMed).

Wang, Zhao, Fan, Gong, Li, Qi, Liu: "Research on the mechanism of Zuogui Pill and Yougui Pill in promoting axonal regeneration in model rats of autoimmune encephalomyelitis." in: Chinese journal of integrative medicine, Vol. 16, Issue 2, pp. 167-72, (2010) (PubMed).

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Andere Bezeichnung: NGF (NGF Produkte)

Hintergrund:

Synonyms: Beta-nerve growth factor,Beta-NGF,NGF,NGFB,

Background: Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. The nucleotide sequence of human and mouse beta-NGF are very similar. The beta-subunits of nerve growth factor (NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception.

Sequence Similarities: Belongs to the NGF-beta family.

Molekulargewicht: 60 kDa

UniProt: P01138

Pathways: Regulation of Cell Size