p63 Antikörper (AA 311-680)

Produktnummer ABIN3043716

Dieses Anti-p63-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von p63 in WB, IHC, ICC, IF und FACS. Geeignet für Human.

Kurzübersicht für p63 Antikörper (AA 311-680) (ABIN3043716)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p63 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-p63 Antikörper

Bindungsspezifität: AA 311-680

Verwendungszweck: Anti-p63/TP63 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-p63/TP63 Antibody Picoband® (ABIN3043716). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human p63 recombinant protein (Position: S311-E680). Human p63 shares 98% amino acid (aa) sequence identity with both mouse and rat p63.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Yang A, Kaghad M, Wang Y, Gillett E, Fleming MD, Dötsch V, Andrews NC, Caput D, McKeon F (September 1998). "p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities". Mol. Cell 2 (3): 305-16. 2. Osada M, Ohba M, Kawahara C, Ishioka C, Kanamaru R, Katoh I, Ikawa Y, Nimura Y, Nakagawara A, Obinata M, Ikawa S (July 1998). "Cloning and functional analysis of human p51, which structurally and functionally resembles p53". Nat. Med. 4 (7): 839-43. 3. Crum CP, McKeon FD (2010). "p63 in epithelial survival, germ cell surveillance, and neoplasia". Annu Rev Pathol 5: 349-71.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Andere Bezeichnung: TP63

Hintergrund:

Synonyms: Tumor protein 63,p63,Chronic ulcerative stomatitis protein,CUSP,Keratinocyte transcription factor KET,Transformation-related protein 63,TP63,Tumor protein p73-like,p73L,p40,p51,TP63,KET, P63, P73H, P73L, TP73L,

Tissue Specificity: Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. .

Background: Tumor protein p63 (TP63), also known as the p63 or TP73L is a protein that in humans is encoded by the TP63 gene. It is mapped to 3q28. TP63 is a member of the p53 family of transcription factors. This gene encodes for two main isoforms by alternative promoters (TAp63 and ΔNp63). TP63 has been mostly restricted to its apoptotic function and more recently as the guardian of oocyte integrity. It has been found that the combined loss of TP63 and p73 results in the failure of cells containing functional p53 to undergo apoptosis in response to DNA damage. TP63 is an essential regulator of stem cell maintenance in stratified epithelial tissues. Tp63 is also critical for maintaining the progenitor-cell populations that are necessary to sustain epithelial development and morphogenesis.

Sequence Similarities: Belongs to the p53 family.

Molekulargewicht: 75 kDa

Gen-ID: 8626

UniProt: Q9H3D4