XAF1 Antikörper (C-Term)

Produktnummer ABIN3043705

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch XAF1 in WB, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für XAF1 Antikörper (C-Term) (ABIN3043705)

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser XAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-XAF1 Antikörper

Bindungsspezifität: AA 283-301, C-Term

Verwendungszweck: Anti-XIAP-associated factor 1 XAF1 Antibody Picoband®

Sequenz: QEKCRWLASS KGKQVRNFS

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-XIAP-associated factor 1 XAF1 Antibody (ABIN3043705). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human XAF1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Fong, W. G., Liston, P., Rajcan-Separovic, E., St. Jean, M., Craig, C., Korneluk, R. G. : Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics 70: 113-122, 2000. 2. Liston, P., Fong, W. G., Kelly, N. L., Toji, S., Miyazaki, T., Conte, D., Tamai, K., Craig, C. G., McBurney, M. W., Korneluk, R. G. : Identification of XAF1 as an antagonist of XIAP anti-caspase activity. Nature Cell Biol. 3: 128-133, 2001.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Andere Bezeichnung: XAF1

Hintergrund:

Synonyms: XIAP-associated factor 1,BIRC4-binding protein,XAF1,BIRC4BP, XIAPAF1,

Tissue Specificity: Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level). .

Background: XIAP associated factor-1, also known as XAF1, is a human gene. X-linked inhibitor of apoptosis (XIAP, MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP (BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. And alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Sequence Similarities: Contains 1 TRAF-type zinc finger.

Molekulargewicht: 35 kDa