Annexin a1 Antikörper (N-Term)

Produktnummer ABIN3043680

Dieses Anti-Annexin a1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Annexin a1 in WB, IHC, FACS, ICC und IF. Geeignet für Human und Affe. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für Annexin a1 Antikörper (N-Term) (ABIN3043680)

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Annexin a1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-Annexin a1 Antikörper

Bindungsspezifität: AA 3-24, N-Term

Verwendungszweck: Anti-Annexin A1/ANXA1 Antibody Picoband®

Sequenz: MVSEFLKQAW FIENEEQEYV QT

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Annexin A1/ANXA1 Antibody (ABIN3043680). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin A1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Wallner, B. P., Mattaliano, R. J., Hession, C., Cate, R. L., Tizard, R., Sinclair, L. K., Foeller, C., Chow, E. P., Browning, J. L., Ramachandran, K. L., Pepinsky, R. B. : Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature 320: 77-81, 1986. . 2. Kovacic RT, Tizard R, Cate RL, Frey AZ, Wallner BP. Correlation of gene and protein structure of rat and human lipocortin I. Biochemistry. 1991 Sep 17,30(37):9015-21. 3. Walther, A., Riehemann, K., Gerke, V. A novel ligand of the formyl peptide receptor: annexin I regulates neutrophil extravasation by interacting with the FPR. Molec. Cell 5: 831-840, 2000.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Annexin a1 Antikörper (ABIN3043680)

Zhang, Wu, Zha, Zhao, Feng: "The prognostic value and clinicopathological features of sarcomatoid differentiation in patients with renal cell carcinoma: a systematic review and meta-analysis." in: Cancer management and research, Vol. 10, pp. 1687-1703, (2018) (PubMed).

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." in: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Mohamed, Idris, Ahmed, Åstrøm, Mustafa, Ibrahim, Mustafa: "Influence of type 2 diabetes on local production of inflammatory molecules in adults with and without chronic periodontitis: a cross-sectional study." in: BMC oral health, Vol. 15, pp. 86, (2015) (PubMed).

Details zu Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Andere Bezeichnung: ANXA1

Hintergrund:

Synonyms: Annexin A1,Annexin I,Annexin-1,Calpactin II,Calpactin-2,Chromobindin-9,Lipocortin I,Phospholipase A2 inhibitory protein,p35,ANXA1,ANX1, LPC1,

Tissue Specificity: Widely expressed, with highest expression in brain. .

Background: Annexin I, also known as lipocortin I (Lipo1), belongs to the family of annexins. These proteins are though to control the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes. In two lipocortins (I and II) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are dependent on its phosphorylation state. The gene in the mouse encodes a protein of 346 amino acid residues. Mouse Lipo1 gene spans about 17 kb and is divided into 13 exons. Annexin I gene, mapped to 9q11-q22, is located on mouse chromosome 19. Annexin I acts through the formyl peptide receptor on human neutrophils. Peptides derived from the unique N-terminal domain of annexin I serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner.

Sequence Similarities: Belongs to the annexin family.

Molekulargewicht: 39 kDa

UniProt: P04083

Pathways: Hormone Transport