Aquaporin 1 Antikörper (C-Term)

Produktnummer ABIN3043524

Dieses Anti-Aquaporin 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Aquaporin 1 in WB, IHC, IF und FACS. Geeignet für Human, Ratte und Maus. Dieses Primary Antibody wurde in 6+ Publikationen zitiert.

Kurzübersicht für Aquaporin 1 Antikörper (C-Term) (ABIN3043524)

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aquaporin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-Aquaporin 1 Antikörper

Bindungsspezifität: AA 240-269, C-Term

Verwendungszweck: Anti-Aquaporin 1/AQP1 Antibody Picoband®

Sequenz: DRVKVWTSGQ VEEYDLDADD INSRVEMKPK

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Aquaporin 1/AQP1 Antibody Picoband® (ABIN3043524). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 1, different from the related mouse and rat sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human1. Denker, B. M., Smith, B. L., Kuhajda, F. P., Agre, P. : Identification, purification, and partial characterization of a novel M(r) 28,000 integral membrane protein from erythrocytes and renal tubules. J. Biol. Chem. 263: 15634-15642, 1988. 2. Thiagarajah, J. R., Verkman, A. S. : Aquaporin deletion in mice reduces corneal water permeability and delays restoration of transparency after swelling. J. Biol. Chem. 277: 19139-19144, 2002.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Aquaporin 1 Antikörper (ABIN3043524)

Zhang, Chen, Dong, Shi: "Effect of selective inhibition of aquaporin 1 on chemotherapy sensitivity of J82 human bladder cancer cells." in: Oncology letters, Vol. 15, Issue 3, pp. 3864-3869, (2018) (PubMed).

Li, Zhao, Miao, Xu, Xiao, Liu: "Dynamic expression and roles of sequestome‑1/p62 in LPS‑induced acute kidney injury in mice." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 7618-7626, (2018) (PubMed).

Wang, Bu, Zhang, Chen, Zhang, Bao: "Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5625-32, (2016) (PubMed).

Huo, Liu, Wang, Zhang, Wang, Yang, Sun, Xu: "Early hyperbaric oxygen therapy inhibits aquaporin 4 and adrenocorticotropic hormone expression in the pituitary gland of rabbits with blast-induced craniocerebral injury." in: Neural regeneration research, Vol. 7, Issue 22, pp. 1729-35, (2015) (PubMed).

Zhang, Wu, Yang, Liu, Liu: "Effects of dexmedetomidine on the protection of hyperoxia-induced lung injury in newborn rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 6, pp. 6466-73, (2015) (PubMed).

Ran, Wang, Chen, Zeng, Zhou, Zheng, Sun, Wang, Lv, Liang, Zhang, Liu: "Aquaporin-1 expression and angiogenesis in rabbit chronic myocardial ischemia is decreased by acetazolamide." in: Heart and vessels, Vol. 25, Issue 3, pp. 237-47, (2010) (PubMed).

Details zu Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Andere Bezeichnung: AQP1

Hintergrund:

Synonyms: Aquaporin-1,AQP-1,Aquaporin-CHIP,Urine water channel,Water channel protein for red blood cells and kidney proximal tubule,AQP1,CHIP28,

Tissue Specificity: Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. .

Background: Aquaporin 1 is a 28-kD integral protein thought at first to be a breakdown product of the Rh polypeptide but was later shown to be a unique molecule that is abundant in erythrocytes and renal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the ion of an osmotic gradient.

Sequence Similarities: Belongs to the MIP/aquaporin (TC 1.A.8) family.

Molekulargewicht: 25, 35-38 kDa

Gen-ID: 358

UniProt: P29972

Pathways: Hormone Transport