CD8 alpha Antikörper (AA 22-187)

Produktnummer ABIN3043499

Der Kaninchen Polyklonal Anti-CD8 alpha-Antikörper wurde für IHC und WB validiert. Er ist geeignet, CD8 alpha in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für CD8 alpha Antikörper (AA 22-187) (ABIN3043499)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD8 alpha Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails anti-CD8 alpha Antikörper

Bindungsspezifität: AA 22-187

Verwendungszweck: Anti-CD8 alpha/CD8A Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-CD8 alpha/CD8A Antibody Picoband® (ABIN3043499). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Bowcock, A. M., Kavathas, P., Margolskee, R. F., Herzenberg, L., Cavalli-Sforza, L. L. An RFLP associated with pcDLeu2-14, a human T-cell differentiation antigen CD8 (Leu2) cDNA mapped to 2p12. Nucleic Acids Res. 14: 7817 only, 1986. 2. "Entrez Gene: CD8A CD8a molecule"

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-CD8 alpha Antikörper (ABIN3043499)

Xu, Chen, Zhao, Huang, Duan, Tong, Zhang, Li, Chang, Chen: "The CD8? gene in duck (Anatidae): cloning, characterization, and expression during viral infection." in: Molecular biology reports, Vol. 42, Issue 2, pp. 431-9, (2015) (PubMed).

Xu, Tan, Wang, Wang, Li, Wu, Chen, Wu, Tan: "Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion." in: International journal of molecular medicine, Vol. 35, Issue 6, pp. 1501-10, (2015) (PubMed).

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, (2005) (PubMed).

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8A

Hintergrund:

Synonyms: T-cell surface glycoprotein CD8 alpha chain,T-lymphocyte differentiation antigen T8/Leu-2,CD8a,CD8A,MAL,

Tissue Specificity: Brain.

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Sequence Similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Molekulargewicht: 55 kDa

Gen-ID: 925

UniProt: P01732

Pathways: T-Zell Rezeptor Signalweg