BMP2 Antikörper (C-Term)

Produktnummer ABIN3043489

Produktdetails anti-BMP2 Antikörper

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Bindungsspezifität: AA 283-312, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BMP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Verwendungszweck: Anti-BMP2 Antibody Picoband®

Sequenz: QAKHKQRKRL KSSCKRHPLY VDFSDVGWND

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-BMP2 Antibody Picoband® (ABIN3043489). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human BMP-2, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
ELISA(Cap) , 1-5 μg/mL, Human, -
1. Gopal Rao, V. V. N., Loffler, C., Wozney, J. M., Hansmann, I. The gene for bone morphogenetic protein 2A (BMP2A) is localized to human chromosome 20p12 by radioactive and nonradioactive in situ hybridization. Hum. Genet. 90: 299-302, 1992.  2. Kaplan, F. S., Tabas, J. A., Zasloff, M. A. Fibrodysplasia ossificans progressiva: a clue from the fly? Calcif. Tissue Int. 47: 117-125, 1990.  3. Sampath TK, Coughlin JE, Whetstone RM, Banach D, Corbett C, Ridge RJ, Ozkaynak E, Oppermann H, Rueger DC (August 1990)."Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily". J. Biol. Chem. 265 (22): 13198-205.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-BMP2 Antikörper (ABIN3043489)

Jian, Fan, Hu, He, Li, Zheng, Ren, Li: "Effects of rhBMP-2 gene transfection to periodontal ligament cells on osteogenesis." in: Bioscience reports, Vol. 37, Issue 3, (2018) (PubMed).

Fernández, Córdoba: "Hyaluronic acid-induced capacitation involves protein kinase C and tyrosine kinase activity modulation with a lower oxidative metabolism in cryopreserved bull sperm." in: Theriogenology, Vol. 122, pp. 68-73, (2018) (PubMed).

Xu, Sun, Zhang, Xu, Du, Zhang, Wang: "Diabetes mellitus affects the biomechanical function of the callus and the expression of TGF-beta1 and BMP2 in an early stage of fracture healing." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 49, Issue 1, pp. e4736, (2016) (PubMed).

Liu, Chen, Zeng, Cui, Ning, Wang, Belguise, Wang, Qian, Lu, Yi: "Bone morphogenic protein-2 regulates the myogenic differentiation of PMVECs in CBDL rat serum-induced pulmonary microvascular remodeling." in: Experimental cell research, Vol. 336, Issue 1, pp. 109-18, (2015) (PubMed).

Wang, Xue, Zhao, Liu, Ma, Ma: "Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera." in: International journal of ophthalmology, Vol. 8, Issue 1, pp. 39-45, (2015) (PubMed).

Chai, Guo, Wang, Gao, Liu, Fu, Guan, Tan, Yang: "In vitro and in vivo evaluations on osteogenesis and biodegradability of a β-tricalcium phosphate coated magnesium alloy." in: Journal of biomedical materials research. Part A, Vol. 100, Issue 2, pp. 293-304, (2014) (PubMed).

Guo, Zeng, Yan, Shen, Liu, Li, Zhang, Wu, Guan, Huang: "Proliferative effect and osteoinductive potential of extracellular matrix coated on cell culture plates." in: SpringerPlus, Vol. 2, Issue 1, pp. 303, (2014) (PubMed).

Sun, Sun, Tian, Xu, Zhou, Xi, Yan, Wang: "A comparison of osteocyte bioactivity in fine particulate bone powder grafts vs larger bone grafts in a rat bone repair model." in: Acta histochemica, Vol. 116, Issue 6, pp. 1015-21, (2014) (PubMed).

Zhong, Liu, Dai, Zhou, Fu: "Sodium thiosulfate protects human aortic smooth muscle cells from osteoblastic transdifferentiation via high-level phosphate." in: The Kaohsiung journal of medical sciences, Vol. 29, Issue 11, pp. 587-93, (2013) (PubMed).

Qiao, Zhang, Jin, Miao, Shi, Liu, Yuan, Liu, Li, Zheng, Zhang, Li, Yang, Sun: "Using poly(lactic-co-glycolic acid) microspheres to encapsulate plasmid of bone morphogenetic protein 2/polyethylenimine nanoparticles to promote bone formation in vitro and in vivo." in: International journal of nanomedicine, Vol. 8, pp. 2985-95, (2013) (PubMed).

Li, Chen, Wu, Jiang, Ge, Gao, Zhang, Wu: "Enhancement of the osseointegration of a polyethylene terephthalate artificial ligament graft in a bone tunnel using 58S bioglass." in: International orthopaedics, Vol. 36, Issue 1, pp. 191-7, (2012) (PubMed).

Wang, Liu, Dang, Ma, Zhang, Wang: "The effect of core decompression on local expression of BMP-2, PPAR-γ and bone regeneration in the steroid-induced femoral head osteonecrosis." in: BMC musculoskeletal disorders, Vol. 13, pp. 142, (2012) (PubMed).

Chen, Kong, Wan, Xiao, Li, Wang, Lin, Wang: "Effects of huogu I formula (I) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head." in: Chinese journal of integrative medicine, Vol. 18, Issue 5, pp. 378-84, (2012) (PubMed).

Liao, Chen, Li, Ge, Duan, Zou, Ge: "Transfer of bone-marrow-derived mesenchymal stem cells influences vascular remodeling and calcification after balloon injury in hyperlipidemic rats." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 165296, (2012) (PubMed).

Yao, Wang, Wang, Wang, Zhang, Liu: "Synergistic enhancement of new bone formation by recombinant human bone morphogenetic protein-2 and osteoprotegerin in trans-sutural distraction osteogenesis: a pilot study in dogs." in: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, Vol. 69, Issue 11, pp. e446-55, (2011) (PubMed).

Han, Li, Guan: "Ectopic osteogenesis of hBMP-2 gene-transduced human bone mesenchymal stem cells/BCB." in: Connective tissue research, Vol. 51, Issue 4, pp. 274-81, (2010) (PubMed).

Liu, Zhong, Liang, Fu, Luo, Zhou, Gou, Huang: "Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway." in: Journal of Zhejiang University. Science. B, Vol. 11, Issue 12, pp. 905-11, (2010) (PubMed).

Ma, Ma, Guo, Zhang: "Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer patients." in: Journal of experimental & clinical cancer research : CR, Vol. 29, pp. 85, (2010) (PubMed).

Tian, Sun, Zhang, Gao, Fu, Yang: "Construction and expression of a bicistronic vector containing human bone morphogenetic protein 2 and vascular endothelial growth factor-165 genes in vitro." in: Chinese medical journal, Vol. 122, Issue 4, pp. 471-3, (2009) (PubMed).

Hu, Cui, Yang, Wang, Hu, Li, Zeng: "Bone morphogenetic protein-2: a potential regulator in scleral remodeling." in: Molecular vision, Vol. 14, pp. 2373-80, (2008) (PubMed).

Details zu BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Andere Bezeichnung: BMP2 (BMP2 Produkte)

Hintergrund:

Synonyms: Bone morphogenetic protein 2,BMP-2,Bone morphogenetic protein 2A,BMP-2A,BMP2,BMP2A,

Tissue Specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Background: BMP2 is also known as Bone morphogenetic protein 2 or BMP2A. It is mapped to 20p12. The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily.?BMP-2, like other?bone morphogenetic proteins,?plays an important role in the development of bone and cartilage. It is involved in the?hedgehog pathway,?TGF beta signaling pathway, and in?cytokine-cytokine receptor interaction. Also, it is involved in cardiac cell differentiation and?epithelial to mesenchymal transition. In addition, BMP2A has been suggested as a reasonable candidate for the human condition fibrodysplasia (myositis) ossificans progressiva, on the basis of observations in a Drosophila model.

Molekulargewicht: 20 kDa, 40 kDa

Gen-ID: 650

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation