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EIF6 Antikörper (AA 66-210)

Der Kaninchen Polyklonal Anti-EIF6-Antikörper wurde für WB, IHC und ICC validiert. Er ist geeignet, EIF6 in Proben von Human, Maus und Ratte zu detektieren.
Produktnummer ABIN3043427

Kurzübersicht für EIF6 Antikörper (AA 66-210) (ABIN3043427)

Target

Alle EIF6 Antikörper anzeigen
EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Reaktivität

  • 37
  • 20
  • 19
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 34
  • 3
Kaninchen

Klonalität

  • 35
  • 2
Polyklonal

Konjugat

  • 26
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser EIF6 Antikörper ist unkonjugiert

Applikation

  • 24
  • 13
  • 9
  • 6
  • 5
  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 66-210

    Verwendungszweck

    Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband® (ABIN3043427). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunocytochemistry, 0.5-1 μg/mL
    Western blot, 0.1-0.5 μg/mL
    1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003. 2. Gandin, V., Miluzio, A., Barbieri, A. M., Beugnet, A., Kiyokawa, H., Marchisio, P. C., Biffo, S. Eukaryotic initiation factor 6 is rate-limiting in translation, growth and transformation. Nature 455: 684-688, 2008. 3. Sanvito, F., Arrigo, G., Zuffardi, O., Agnelli, M., Marchisio, P. C., Biffo, S. Localization of p27 beta-4 binding protein gene (ITGB4BP) to human chromosome region 20q11.2. Genomics 52: 111-112, 1998.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

    Andere Bezeichnung

    EIF6

    Hintergrund

    Synonyms: Eukaryotic translation initiation factor 6 ,eIF-6 ,B (2)GCN homolog,B4 integrin interactor,CAB,p27 (BBP),EIF6 ,EIF3A, ITGB4BP ,OK/SW-cl.27,

    Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .

    Background: EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50 % reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

    Sequence Similarities: Belongs to the WD repeat ESC family.

    Molekulargewicht

    26 kDa

    Gen-ID

    3692

    UniProt

    P56537

    Pathways

    Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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