Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

EIF6 Antikörper (AA 66-210)

EIF6 Reaktivität: Human, Maus, Ratte WB, IHC, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3043427
  • Target Alle EIF6 Antikörper anzeigen
    EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))
    Bindungsspezifität
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 66-210
    Reaktivität
    • 35
    • 20
    • 18
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 33
    • 3
    Kaninchen
    Klonalität
    • 34
    • 2
    Polyklonal
    Konjugat
    • 25
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser EIF6 Antikörper ist unkonjugiert
    Applikation
    • 23
    • 13
    • 9
    • 6
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Verwendungszweck
    Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband® (ABIN3043427). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.
    Isotyp
    IgG
  • Applikationshinweise
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunocytochemistry, 0.5-1 μg/mL
    Western blot, 0.1-0.5 μg/mL
    1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003. 2. Gandin, V., Miluzio, A., Barbieri, A. M., Beugnet, A., Kiyokawa, H., Marchisio, P. C., Biffo, S. Eukaryotic initiation factor 6 is rate-limiting in translation, growth and transformation. Nature 455: 684-688, 2008. 3. Sanvito, F., Arrigo, G., Zuffardi, O., Agnelli, M., Marchisio, P. C., Biffo, S. Localization of p27 beta-4 binding protein gene (ITGB4BP) to human chromosome region 20q11.2. Genomics 52: 111-112, 1998.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))
    Andere Bezeichnung
    EIF6 (EIF6 Produkte)
    Hintergrund

    Synonyms: Eukaryotic translation initiation factor 6 ,eIF-6 ,B (2)GCN homolog,B4 integrin interactor,CAB,p27 (BBP),EIF6 ,EIF3A, ITGB4BP ,OK/SW-cl.27,

    Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .

    Background: EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50 % reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

    Sequence Similarities: Belongs to the WD repeat ESC family.

    Molekulargewicht
    26 kDa
    Gen-ID
    3692
    UniProt
    P56537
    Pathways
    Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
Sie sind hier:
Chat with us!