KIF2C Antikörper (AA 531-725)

Produktnummer ABIN3043278

Dieses Anti-KIF2C-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von KIF2C in WB, IF, IHC, ICC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für KIF2C Antikörper (AA 531-725) (ABIN3043278)

Target: KIF2C (Kinesin Family Member 2C (KIF2C))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIF2C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-KIF2C Antikörper

Bindungsspezifität: AA 531-725

Verwendungszweck: Anti-MCAK/KIF2C Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-MCAK/KIF2C Antibody Picoband® (ABIN3043278). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MCAK recombinant protein (Position: G531-Q725). Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Manning, A. L., Ganem, N. J., Bakhoum, S. F., Wagenbach, M., Wordeman, L., Compton, D. A. The kinesin-13 proteins, Kif2a, Kif2b, and Kif2c/MCAK have distinct roles during mitosis in human cells. Molec. Biol. Cell 18: 2970-2979, 2007. 2. Kim IG, Jun DY, Sohn U, Kim YH (Feb 1998). "Cloning and expression of human mitotic centromere-associated kinesin gene". Biochim Biophys Acta 1359 (3): 181-6. 3. "Entrez Gene: KIF2C kinesin family member 2C"

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu KIF2C

Target: KIF2C (Kinesin Family Member 2C (KIF2C))

Andere Bezeichnung: KIF2C

Hintergrund:

Synonyms: Kinesin-like protein KIF2C,Kinesin-like protein 6,Mitotic centromere-associated kinesin,MCAK,KIF2C,KNSL6,

Tissue Specificity: Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary, expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. .

Background: Kinesin-like protein KIF2C is a protein that in humans is encoded by the KIF2C gene. It is mapped to 1p34.1. The protein encoded by this gene is a member of kinesin-like protein family. Most proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. KIF2C uses microtubule depolymerizing activity to correct improper microtubule attachments at kinetochores.

Sequence Similarities: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.

Molekulargewicht: 81 kDa

Gen-ID: 11004

UniProt: Q99661

Pathways: Microtubule Dynamics