DDT Antikörper (C-Term)

Produktnummer ABIN3043212

Produktdetails anti-DDT Antikörper

Target: DDT (D-Dopachrome Tautomerase (DDT))

Bindungsspezifität: AA 99-118, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Anti-D-dopachrome decarboxylase DDT Antibody Picoband®

Sequenz: RFFPLESWQI GKIGTVMTFL

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-D-dopachrome decarboxylase DDT Antibody (ABIN3043212). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DDT, different from the related mouse and rat sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
1. Esumi, N., Budarf, M., Ciccarelli, L., Sellinger, B., Kozak, C. A., Wistow, G.Conserved gene structure and genomic linkage for D-dopachrome tautomerase (DDT) and MIF.Mammalian Genome 9: 753-757, 1998. 2. Nishihira, J., Fujinaga, M., Kuriyama, T., Suzuki, M., Sugimoto, H., Nakagawa, A., Tanaka, I., Sakai, M.Molecular cloning of human D-dopachrome tautomerase cDNA: N-terminal proline is essential for enzyme activation.Biochem. Biophys. Res. Commun. 243: 538-544, 1998.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu DDT

Target: DDT (D-Dopachrome Tautomerase (DDT))

Andere Bezeichnung: DDT (DDT Produkte)

Hintergrund:

Synonyms: D-dopachrome decarboxylase,4.1.1.84,D-dopachrome tautomerase,Phenylpyruvate tautomerase II,DDT,

Tissue Specificity: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.

Sequence Similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Molekulargewicht: 13-15 kDa

UniProt: P30046