HMGB4 Antikörper (N-Term)

Produktnummer ABIN3043021

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HMGB4 in WB. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für HMGB4 Antikörper (N-Term) (ABIN3043021)

Target: HMGB4 (High Mobility Group Box 4 (HMGB4))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGB4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-HMGB4 Antikörper

Bindungsspezifität: AA 24-41, N-Term

Verwendungszweck: Anti-High mobility group protein B4 HMGB4 Antibody Picoband®

Sequenz: RNKFKEQQPN TYVGFKEF

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-High mobility group protein B4 HMGB4 Antibody (ABIN3043021). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB4, different from the related mouse and rat sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Phosphorylation of maize and Arabidopsis HMGB proteins by protein kinase CK2alpha.Stemmer C, Leeming DJ, Franssen L, Grimm R, Grasser KD. Institute of Life Sciences, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-HMGB4 Antikörper (ABIN3043021)

Rouhiainen, Zhao, Vanttola, Qian, Kulesskiy, Kuja-Panula, Gransalke, Grönholm, Unni, Meistrich, Tian, Auvinen, Rauvala: "HMGB4 is expressed by neuronal cells and affects the expression of genes involved in neural differentiation." in: Scientific reports, Vol. 6, pp. 32960, (2018) (PubMed).

Zhao, Xue, Wang: "HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 6138-6146, (2017) (PubMed).

Details zu HMGB4

Target: HMGB4 (High Mobility Group Box 4 (HMGB4))

Andere Bezeichnung: HMGB4

Hintergrund:

Synonyms: High mobility group protein B4,HMGB4,

Tissue Specificity: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .

Background: HMGB4 is HMG2 like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, which is found in a variety of eukaryotic chromosomal proteins and transcription. HMGB proteins are phosphorylated to various extents reveals that the existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions.

Sequence Similarities: Belongs to the HMGB family.

Molekulargewicht: 23 kDa