OSBP Antikörper (Middle Region)

Produktnummer ABIN3042983

Der Kaninchen Polyklonal Anti-OSBP-Antikörper wurde für WB validiert. Er ist geeignet, OSBP in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für OSBP Antikörper (Middle Region) (ABIN3042983)

Target: OSBP (Oxysterol Binding Protein (OSBP))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OSBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-OSBP Antikörper

Bindungsspezifität: AA 290-304, Middle Region

Verwendungszweck: Anti-OSBP1 Antibody Picoband®

Sequenz: WQKSLQYERD QRIRL

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-OSBP1 Antibody (ABIN3042983). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human OSBP1, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Im, Y. J., Raychaudhuri, S., Prinz, W. A., Hurley, J. H. Structural mechanism for sterol sensing and transport by OSBP-related proteins. Nature 437: 154-158, 2005. 2. Moreira, E. F., Jaworski, C., Li, A., Rodriguez, I. R. Molecular and biochemical characterization of a novel oxysterol-binding protein (OSBP2) highly expressed in retina. J. Biol. Chem. 276: 18570-18578, 2001. 3. Wang, P., Weng, J., Anderson, R. G. W. OSBP is a cholesterol-regulated scaffolding protein in control of ERK1/2 activation. Science 307: 1472-1476, 2005.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu OSBP

Target: OSBP (Oxysterol Binding Protein (OSBP))

Andere Bezeichnung: OSBP

Hintergrund:

Synonyms: Oxysterol-binding protein 1,OSBP,OSBP1,

Tissue Specificity: Widely expressed. .

Background: OSBP (Oxysterol-Binding Protein), also called OSBP1, is a protein that in humans is encoded by the OSBP gene. Im et al. (2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein (ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al. (2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al. (2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase (ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.

Sequence Similarities: Belongs to the OSBP family.

Molekulargewicht: 75 kDa

UniProt: P22059