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Flotillin 1 Antikörper (Middle Region)

FLOT1 Reaktivität: Human, Maus, Ratte WB, IHC, IP Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3042941
  • Target Alle Flotillin 1 (FLOT1) Antikörper anzeigen
    Flotillin 1 (FLOT1)
    Bindungsspezifität
    • 8
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 219-234, Middle Region
    Reaktivität
    • 51
    • 27
    • 24
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 49
    • 2
    • 2
    Kaninchen
    Klonalität
    • 40
    • 13
    Polyklonal
    Konjugat
    • 26
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Dieser Flotillin 1 Antikörper ist unkonjugiert
    Applikation
    • 31
    • 20
    • 11
    • 8
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Verwendungszweck
    Anti-Flotillin 1/FLOT1 Antibody Picoband®
    Sequenz
    KKAAYDIEVN TRRAQA
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-Flotillin 1/FLOT1 Antibody (ABIN3042941). Tested in IP, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence in the middle region of human Flotillin 1, different from the related rat and mouse sequences by one amino acid.
    Isotyp
    IgG
    Top Product
    Discover our top product FLOT1 Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry, 2-5 μg/mL, Human
    Immunoprecipitation, 0.5-2 μg/mL, Human
    1. Bickel, P. E., Scherer, P. E., Schnitzer, J. E., Oh, P., Lisanti, M. P., Lodish, H. F. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J. Biol. Chem. 272: 13793-13802, 1997. 2. Hazarika, P., Dham, N., Patel, P., Cho, M., Weidner, D., Goldsmith, L., Duvic, M. Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation. J. Cell. Biochem. 75: 147-159, 1999. 3. Santamaria, A., Castellanos, E., Gomez, V., Benedit, P., Renau-Piqueras, J., Morote, J., Reventos, J., Thomson, T. M., Paciucci, R. PTOV1 enables the nuclear translocation and mitogenic activity of flotillin-1, a major protein of lipid rafts. Molec. Cell. Biol. 25: 1900-1911, 2005.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Shi, Li, Liu, Yin, Zhao: "β-Cyclodextrin induces the differentiation of resident cardiac stem cells to cardiomyocytes through autophagy." in: Biochimica et biophysica acta. Molecular cell research, Vol. 1864, Issue 8, pp. 1425-1434, (2017) (PubMed).

  • Target
    Flotillin 1 (FLOT1)
    Andere Bezeichnung
    FLOT1 (FLOT1 Produkte)
    Hintergrund

    Synonyms: Flotillin-1,FLOT1,

    Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .

    Background: FLOT1 (Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al. (1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al. (1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al. (2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.

    Sequence Similarities: Belongs to the eIF-3 subunit B family.

    Molekulargewicht
    47 kDa
    UniProt
    O75955
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