PFN2 Antikörper (C-Term)

Produktnummer ABIN3042861

Produktdetails anti-PFN2 Antikörper

Target: PFN2 (Profilin 2 (PFN2))

Bindungsspezifität: AA 125-140, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PFN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Profilin-2(PFN2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: NKKAYSMAKY LRDSGF

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Profilin-2(PFN2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: profilin 2
Protein Name: Profilin-2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 2(125-140aa NKKAYSMAKYLRDSGF), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu PFN2

Target: PFN2 (Profilin 2 (PFN2))

Andere Bezeichnung: PFN2 (PFN2 Produkte)

Synonyme: D3S1319E antikoerper, PFL antikoerper, Pfn antikoerper, Profilin2 antikoerper, XProfilin2 antikoerper, PFN2 antikoerper, PRO2 antikoerper, Profilin-2 antikoerper, pfl antikoerper, d3s1319e antikoerper, profilin-2 antikoerper, F17A13.170 antikoerper, F17A13_170 antikoerper, PRF2 antikoerper, PROFILIN antikoerper, PROFILIN 2 antikoerper, profilin 2 antikoerper, wu:fb01g08 antikoerper, wu:fb54f09 antikoerper, wu:fd58f06 antikoerper, profilin 2 antikoerper, profilin 2 L homeolog antikoerper, profilin homolog 2 antikoerper, Profilin-2 antikoerper, PFN2 antikoerper, Pfn2 antikoerper, pfn2.L antikoerper, prf2 antikoerper, pfn2 antikoerper, pfn-2 antikoerper

Hintergrund: Profilin-2, also called PFN2, is a protein that in humans is encoded by the PFN2 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. This gene is mapped to 3q25.1. It is though to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. It binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Synonyms: D3S1319E antibody|PFL antibody|PFN 2 antibody|PFN2 antibody|PROF2_HUMAN antibody|Profilin II antibody|Profilin-2 antibody|Profilin2 antibody|ProfilinII antibody

UniProt: P35080

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis