TIA1 Antikörper (N-Term)

Produktnummer ABIN3042843

Dieses Anti-TIA1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TIA1 in WB. Geeignet für Human und Maus.

Kurzübersicht für TIA1 Antikörper (N-Term) (ABIN3042843)

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TIA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-TIA1 Antikörper

Bindungsspezifität: AA 98-110, N-Term

Verwendungszweck: Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody Picoband®

Sequenz: STQRSQDHFH VFV

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody (ABIN3042843). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human TIA1, identical to the related mouse sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Hackman, P., Sarparanta, J., Lehtinen, S., Vihola, A., Evila, A., Jonson, P. H., Luque, H., Kere, J., Screen, M., Chinnery, P. F., Ahlberg, G., Edstrom, L., Udd, B. Welander distal myopathy is caused by a mutation in the RNA-binding protein TIA1. Ann. Neurol. 73: 500-509, 2013. 2. Klar, J., Sobol, M., Melberg, A., Mabert, K., Ameur, A., Johansson, A. C. V., Feuk, L., Entesarian, M., Orlen, H., Casar-Borota, O., Dahl, N. Welander distal myopathy caused by an ancient founder mutation in TIA1 associated with perturbed splicing. Hum. Mutat. 34: 572-577, 2013.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Andere Bezeichnung: TIA1

Hintergrund:

Synonyms: Nucleolysin TIA-1 isoform p40,RNA-binding protein TIA-1,T-cell-restricted intracellular antigen-1,TIA-1,p40-TIA-1,TIA1,

Tissue Specificity: Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined.

Background: TIA1, also called WDM, encodes an RNA-binding protein involved in splicing regulation and translational repression. The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. By in situ hybridization, this gene is mapped to chromosome 2p13.3. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly (A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15- kDa protein that is though to be derived from the carboxyl terminus of the 40- kDa product by proteolytic processing.

Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Molekulargewicht: 274 kDa

UniProt: P31483