Cathepsin D Antikörper (AA 65-410)

Produktnummer ABIN3042775

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Cathepsin D in WB, IHC, ICC und IF. Er zeigt eine Reaktivität gegenüber Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für Cathepsin D Antikörper (AA 65-410) (ABIN3042775)

Target: Cathepsin D (CTSD)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cathepsin D Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-Cathepsin D Antikörper

Bindungsspezifität: AA 65-410

Verwendungszweck: Anti-Cathepsin D/CTSD Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Cathepsin D/CTSD Antibody Picoband® (ABIN3042775). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Cathepsin D recombinant protein (Position: E65-L410). Mouse Cathepsin D shares 85% and 91% amino acid (aa) sequences identity with human and rat Cathepsin D, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
1. Faust PL, Kornfeld S, Chirgwin JM (Sep 1985). "Cloning and sequence analysis of cDNA for human cathepsin D". Proc Natl Acad Sci U S A 82 (15): 4910-4. 2. Wolf M, Clark-Lewis I, Buri C, Langen H, Lis M, Mazzucchelli L (April 2003). "Cathepsin D specifically cleaves the chemokines macrophage inflammatory protein-1 alpha, macrophage inflammatory protein-1 beta, and SLC that are expressed in human breast cancer". Am. J. Pathol. 162 (4): 1183-90.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Cathepsin D Antikörper (ABIN3042775)

Zheng, Li, Wang, Wang, Liao, Hu, Fan, Kang, Zeng, Wu, Wu, Zhang, Wang, He et al.: "Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage ..." in: Autophagy, Vol. 12, Issue 9, pp. 1593-613, (2017) (PubMed).

Details zu Cathepsin D

Target: Cathepsin D (CTSD)

Andere Bezeichnung: CTSD

Hintergrund:

Synonyms: Cathepsin D,3.4.23.5,Ctsd,

Tissue Specificity: Expressed in the aorta extrcellular space (at protein level). .

Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Sequence Similarities: Belongs to the peptidase A1 family.

Molekulargewicht: 45 kDa

Gen-ID: 13033

UniProt: P18242

Pathways: Peptide Hormone Metabolism