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Cofilin Antikörper (AA 2-166)

CFL1 Reaktivität: Human, Maus, Ratte, Affe WB, ELISA, IHC, IF, FACS, ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3042747
  • Target Alle Cofilin (CFL1) Antikörper anzeigen
    Cofilin (CFL1) (Cofilin 1 (CFL1))
    Bindungsspezifität
    • 16
    • 15
    • 14
    • 11
    • 8
    • 7
    • 7
    • 7
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-166
    Reaktivität
    • 113
    • 75
    • 72
    • 6
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Affe
    Wirt
    • 117
    • 8
    • 2
    Kaninchen
    Klonalität
    • 116
    • 10
    Polyklonal
    Konjugat
    • 63
    • 9
    • 9
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Cofilin Antikörper ist unkonjugiert
    Applikation
    • 64
    • 54
    • 44
    • 31
    • 31
    • 28
    • 28
    • 19
    • 18
    • 8
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Verwendungszweck
    Anti-Cofilin/CFL1 Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-Cofilin/CFL1 Antibody Picoband® (ABIN3042747). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.
    Isotyp
    IgG
    Top Product
    Discover our top product CFL1 Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Cooper, G. M. and R. E. Hausman. The Cell: A Molecular Approach, 3rd ed. Washington DC: ASM Press 2004 pp.436-440. 2. Ichetovkin I, Han J, Pang KM, Knecht DA, Condeelis JS (April 2000). "Actin filaments are severed by both native and recombinant dictyostelium cofilin but to different extents". Cell Motil. Cytoskeleton 45 (4): 293-306. 3. Lappalainen P, Drubin DG (July 1997). "Cofilin promotes rapid actin filament turnover in vivo".Nature 388 (6637): 78-82.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    Cofilin (CFL1) (Cofilin 1 (CFL1))
    Andere Bezeichnung
    CFL1 (CFL1 Produkte)
    Hintergrund

    Synonyms: Cofilin-1,18 kDa phosphoprotein,p18,Cofilin, non-muscle isoform,CFL1,CFL,

    Tissue Specificity: Widely distributed in various tissues.

    Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70 % sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH , phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

    Sequence Similarities: Belongs to the actin-binding proteins ADF family.

    Molekulargewicht
    19 kDa
    Gen-ID
    1072
    UniProt
    P23528
    Pathways
    Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
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