Reticulon 3 Antikörper (C-Term)

Produktnummer ABIN3042575

Der Kaninchen Polyklonal anti-Reticulon 3 Antikörper wird verwendet zum Nachweis von Reticulon 3 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für Reticulon 3 Antikörper (C-Term) (ABIN3042575)

Target: Reticulon 3 (RTN3)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Reticulon 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Reticulon 3 Antikörper

Bindungsspezifität: AA 1017-1032, C-Term

Verwendungszweck: Rabbit IgG polyclonal antibody for Reticulon-3(RTN3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: EKIQAKLPGI AKKKAE

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Reticulon-3(RTN3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: reticulon 3
Protein Name: Reticulon-3

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RTN3(1017-1032aa EKIQAKLPGIAKKKAE), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu Reticulon 3

Target: Reticulon 3 (RTN3)

Andere Bezeichnung: RTN3

Hintergrund: Reticulon-3, also known as RTN3 or NSPL2, is a protein that in humans is encoded by the RTN3 gene. It is mapped to 11q13, which is between markers D11S4535 and D11S4627. This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1(BACE1), and the production of amyloid-beta. RTN3 colocalized with BACE1 in neurons of human brain gray matter, and overexpression of RTN3 in vitro inhibited BACE1 activity and decreased amyloid precursor protein processing.

Synonyms: ASYIP antibody|Neuroendocrine specific protein like 2 antibody|Neuroendocrine-specific protein-like 2 antibody|Neuroendocrine-specific protein-like II antibody|NSP like protein II antibody|NSP-like protein 2 antibody|NSP-like protein II antibody|NSPL2 antibody|NSPLII antibody|Reticulon 3 antibody|Reticulon-3 antibody|Reticulon3 antibody|Rtn3 antibody|RTN3_HUMAN antibody

UniProt: O95197