CARM1 Antikörper (Middle Region)

Produktnummer ABIN3042568

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CARM1 in WB, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für CARM1 Antikörper (Middle Region) (ABIN3042568)

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CARM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-CARM1 Antikörper

Bindungsspezifität: AA 267-284, Middle Region

Verwendungszweck: Anti-PRMT4/CARM1 Antibody Picoband®

Sequenz: HLAPFTDEQL YMEQFTKA

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-PRMT4/CARM1 Antibody (ABIN3042568). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PRMT4, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Chen D, Ma H, Hong H, Koh SS, Huang SM, Schurter BT, Aswad DW, Stallcup MR (1999). "Regulation of transcription by a protein methyltransferase". Science 284(5423): 2174-7. 2. El Messaoudi, S. E., Fabbrizio, E., Rodriguez, C., Chuchana, P., Fauquier, L., Cheng, D., Theillet, C., Vandel, L., Bedford, M. T., Sardet, C. Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the cyclin E1 gene. Proc. Nat. Acad. Sci. 103: 13351-13356, 2006. 3. Torres-Padilla ME, Parfitt DE, Kouzarides T, Zernicka-Goetz M (2007). "Histone arginine methylation regulates pluripotency in the early mouse embryo". Nature445 (7124): 214-8.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CARM1

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Andere Bezeichnung: CARM1

Hintergrund:

Synonyms: Histone-arginine methyltransferase CARM1,2.1.1.-,2.1.1.125,Coactivator-associated arginine methyltransferase 1,Protein arginine N-methyltransferase 4,CARM1,PRMT4,

Tissue Specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. .

Background: CARM1 (coactivator-associated arginine methyltransferase 1), also known as PRMT4, is an enzyme encoded by the CARM1 gene found in human beings, as well as many other mammals. This gene is mapped to 19p13.2. CARM1 is a regulator of cyclin E1 and DHFR mRNA expression. Its main function includes catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. CARM1 is a secondary coactivator through its association with p160 family (SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts. This gene also plays an important role in androgen receptors and may play a role in prostate cancer progression.

Sequence Similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family.

Molekulargewicht: 66 kDa

UniProt: Q86X55

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of fat Cell Differentiation