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Otoferlin Antikörper (C-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Otoferlin in IHC und WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.
Produktnummer ABIN3042499

Kurzübersicht für Otoferlin Antikörper (C-Term) (ABIN3042499)

Target

Alle Otoferlin (OTOF) Antikörper anzeigen
Otoferlin (OTOF)

Reaktivität

  • 2
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 2
  • 1
Kaninchen

Klonalität

  • 2
  • 1
Polyklonal

Konjugat

  • 3
Dieser Otoferlin Antikörper ist unkonjugiert

Applikation

  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB)
  • Bindungsspezifität

    • 1
    • 1
    • 1
    AA 1831-1863, C-Term

    Verwendungszweck

    Anti-Otoferlin Antibody Picoband®

    Sequenz

    QIWDADHFSA DDFLGAIELD LNRFPRGAKT AKQ

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Otoferlin Antibody Picoband® (ABIN3042499). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human Otoferlin, identical to the related mouse and rat sequences.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Western blot, 0.1-0.5 μg/mL, Human, Rat
    1. "Entrez Gene: OTOF otoferlin". 2. Rodriguez-Ballesteros M, Reynoso R, Olarte M, Villamar M, Morera C, Santarelli R, Arslan E, Meda C, Curet C, Volter C, Sainz-Quevedo M, Castorina P, Ambrosetti U, Berrettini S, Frei K, Tedin S, Smith J, Cruz Tapia M, Cavalle L, Gelvez N, Primignani P, Gomez-Rosas E, Martin M, Moreno-Pelayo MA, Tamayo M, Moreno-Barral J, Moreno F, del Castillo I (May 2008). "A multicenter study on the prevalence and spectrum of mutations in the otoferlin gene (OTOF) in subjects with nonsyndromic hearing impairment and auditory neuropathy". Hum Mutat 29(6): 823-31. 3. Yasunaga S, Grati M, Cohen-Salmon M, El-Amraoui A, Mustapha M, Salem N, El-Zir E, Loiselet J, Petit C (Apr 1999). "A mutation in OTOF, encoding otoferlin, a FER-1-like protein, causes DFNB9, a nonsyndromic form of deafness". Nat Genet 21 (4): 363-9.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    Otoferlin (OTOF)

    Andere Bezeichnung

    OTOF

    Hintergrund

    Synonyms: Otoferlin,Fer-1-like protein 2,OTOF,FER1L2,

    Tissue Specificity: Isoform 1 and isoform 3 are found in adult brain. Isoform 2 is expressed in the fetus and in adult brain, heart, placenta, skeletal muscle and kidney.

    Background: Otoferlin is a protein that in humans is encoded by the OTOF gene. Mutations in this gene are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has three C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has six C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multipleisoforms have been found for this gene.

    Sequence Similarities: Belongs to the ferlin family.

    Molekulargewicht

    227 kDa

    Gen-ID

    9381

    Pathways

    Sensory Perception of Sound, Synaptic Vesicle Exocytosis
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