HEXB Antikörper (AA 381-556)

Produktnummer ABIN3042442

Produktdetails anti-HEXB Antikörper

Target: HEXB (Hexosaminidase B (Beta Polypeptide) (HEXB))

Bindungsspezifität: AA 381-556

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HEXB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Anti-HEXB Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-HEXB Antibody Picoband® (ABIN3042442). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human HEXB recombinant protein (Position: K381-M556). Human HEXB shares 75% and 73% amino acid (aa) sequences identity with mouse and rat HEXB, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. O'Dowd BF, Quan F, Willard HF, Lamhonwah AM, Korneluk RG, Lowden JA, Gravel RA, Mahuran DJ (Apr 1985). "Isolation of cDNA clones coding for the beta subunit of human beta-hexosaminidase". Proc Natl Acad Sci U S A 82 (4): 1184-8. 2. Korneluk RG, Mahuran DJ, Neote K, Klavins MH, O'Dowd BF, Tropak M, Willard HF, Anderson MJ, Lowden JA, Gravel RA (Jul 1986). "Isolation of cDNA clones coding for the alpha-subunit of human beta-hexosaminidase. Extensive homology between the alpha- and beta-subunits and studies on Tay-Sachs disease". J Biol Chem 261 (18): 8407-13. 3. "Entrez Gene: HEXB hexosaminidase B (beta polypeptide)"

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu HEXB

Target: HEXB (Hexosaminidase B (Beta Polypeptide) (HEXB))

Andere Bezeichnung: HEXB (HEXB Produkte)

Hintergrund:

Synonyms: Beta-hexosaminidase subunit beta,3.2.1.52,Beta-N-acetylhexosaminidase subunit beta,Hexosaminidase subunit B,Cervical cancer proto-oncogene 7 protein,HCC-7,N-acetyl-beta-glucosaminidase subunit beta,Beta-hexosaminidase subunit beta chain B,Beta-hexosaminidase subunit beta chain A,HEXB,HCC7,

Tissue Specificity: Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. .

Background: Beta-hexosaminidase subunit beta (HEXB) is an enzyme that in humans is encoded by the HEXB gene. It is mapped to 5q13.3. HEXB is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). It has been found that HEXB is a peptidoglycan hydrolase and it is involved in restricting mycobacteria growth even before the onset of adaptive immunity.

Sequence Similarities: Belongs to the glycosyl hydrolase 20 family.

Molekulargewicht: 63 kDa

Gen-ID: 3074

UniProt: P07686

Pathways: Sensory Perception of Sound, Glycosaminoglycan Metabolic Process