Flotillin 2 Antikörper (AA 169-344)

Produktnummer ABIN3042393

Der Kaninchen Polyklonal anti-Flotillin 2 Antikörper (ABIN3042393) detektiert spezifisch Flotillin 2 in WB, IHC, IF, ICC, IHC (fro) und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für Flotillin 2 Antikörper (AA 169-344) (ABIN3042393)

Target: Flotillin 2 (FLOT2)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Flotillin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Produktdetails anti-Flotillin 2 Antikörper

Bindungsspezifität: AA 169-344

Verwendungszweck: Anti-Flotillin 2/FLOT2 Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse,rat Flotillin 2.

Produktmerkmale: Anti-Flotillin 2/FLOT2 Antibody. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunohistochemistry (Frozen Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Flotillin 2

Target: Flotillin 2 (FLOT2)

Andere Bezeichnung: FLOT2

Hintergrund:

Background: FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.

Gene Full Name: flotillin 2

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molekulargewicht: 47-50 kDa

Gen-ID: 2319

UniProt: Q14254