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CD20 Antikörper (AA 1-261)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CD20 in FACS, WB, IHC, IF, IHC (fro) und ICC. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.
Produktnummer ABIN3042388

Kurzübersicht für CD20 Antikörper (AA 1-261) (ABIN3042388)

Target

Alle CD20 (MS4A1) Antikörper anzeigen
CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reaktivität

  • 349
  • 67
  • 28
  • 28
  • 20
  • 20
  • 17
  • 6
  • 6
  • 4
  • 3
  • 2
  • 1
Human

Wirt

  • 252
  • 127
  • 6
  • 4
  • 2
  • 1
  • 1
Kaninchen

Klonalität

  • 290
  • 92
  • 8
Polyklonal

Konjugat

  • 154
  • 26
  • 18
  • 15
  • 12
  • 12
  • 12
  • 12
  • 12
  • 12
  • 12
  • 12
  • 6
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD20 Antikörper ist unkonjugiert

Applikation

  • 259
  • 135
  • 116
  • 114
  • 55
  • 53
  • 40
  • 30
  • 21
  • 16
  • 16
  • 16
  • 14
  • 13
  • 11
  • 9
  • 6
  • 5
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 34
    • 16
    • 16
    • 13
    • 8
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-261

    Verwendungszweck

    Anti-CD20/MS4A1 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-CD20/MS4A1 Antibody Picoband® (ABIN3042388). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
    Immunocytochemistry, 0.5-1 μg/mL
    Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106cells
    1. Cragg MS, Walshe CA, Ivanov AO, Glennie MJ (2005). "The biology of CD20 and its potential as a target for mAb therapy". Curr. Dir. Autoimmun. Current ions in Autoimmunity 8: 140-74. 2. Hardy, Richard (2008). "Chapter 7: B Lymphocyte Development and Biology". In Paul, William.Fundamental Immunology (Book) (6th ed.). Philadelphia: Lippincott Williams & Wilkins. pp. 237-269. 3. Kuijpers TW, Bende RJ, Baars PA, Grummels A, Derks IA, Dolman KM, Beaumont T, Tedder TF, van Noesel CJ, Eldering E, van Lier RA (January 2010). "CD20 deficiency in humans results in impaired T cell-independent antibody responses". J. Clin. Invest. 120 (1): 214-22.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Ni, Chen, Zeng, Pei, Du, Tang, Wang, Hu, Zhu, He, Wei, Wang, Ning, Wang, Wang, Ma, Chen, Sun, Tang, Wang, Wang: "In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures." in: Cell research, Vol. 25, Issue 7, pp. 785-800, (2016) (PubMed).

    Yan, Fan, Bai, Hou, Li, Tong: "Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148536, (2016) (PubMed).

  • Target

    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

    Andere Bezeichnung

    MS4A1

    Hintergrund

    Synonyms: B-lymphocyte antigen CD20,B-lymphocyte surface antigen B1,Bp35,Leukocyte surface antigen Leu-16,Membrane-spanning 4-domains subfamily A member 1,CD20,MS4A1,CD20,

    Tissue Specificity: Expressed on B-cells.

    Background: CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What's more, this protein may be involved in the regulation of B-cell activation and proliferation.

    Sequence Similarities: Belongs to the MS4A family.

    Molekulargewicht

    35 kDa

    Gen-ID

    931

    UniProt

    P11836
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