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Crossover junction endonuclease EME1 (EME1) (AA 520-561), (C-Term) Antikörper

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch in WB, IHC, ICC, IHC (fro) und FACS. Er zeigt eine Reaktivität gegenüber Human und Ratte.
Produktnummer ABIN3042370

Kurzübersicht für Crossover junction endonuclease EME1 (EME1) (AA 520-561), (C-Term) Antikörper (ABIN3042370)

Target

Alle Crossover junction endonuclease EME1 (EME1) Antikörper anzeigen
Crossover junction endonuclease EME1 (EME1)

Reaktivität

  • 17
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Human, Ratte

Wirt

  • 14
  • 3
Kaninchen

Klonalität

  • 16
  • 1
Polyklonal

Konjugat

  • 13
  • 2
  • 1
  • 1
Unkonjugiert

Applikation

  • 12
  • 8
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 520-561, C-Term

    Verwendungszweck

    Anti-EME1 Antibody Picoband®

    Sequenz

    DKERQNLLAD IQVRRGEGVT STSRRIGPEL SRRIYLQMTT LQ

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-EME1 Antibody Picoband® (ABIN3042370). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human EME1, different from the related mouse sequence by five amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
    Immunocytochemistry, 0.5-1 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: EME1 essential meiotic endonuclease 1 homolog 1 (S. pombe)". 2. Abraham, J., Lemmers, B., Hande, M. P., Moynahan, M. E., Chahwan, C., Ciccia, A., Essers, J., Hanada, K., Chahwan, R., Khaw, A. K., McPherson, P., Shehabeldin, A., Laister, R., Arrowsmith, C., Kanaar, R., West, S. C., Jasin, M., Hakem, R. Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells. EMBO J. 22: 6137-6147, 2003. 3. Ciccia A, Constantinou A, West SC (Jun 2003). "Identification and characterization of the human mus81-eme1 endonuclease". J Biol Chem 278 (27): 25172-8.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    Crossover junction endonuclease EME1 (EME1)

    Andere Bezeichnung

    EME1

    Hintergrund

    Synonyms: Crossover junction endonuclease EME1,3.1.22.-,MMS4 homolog,hMMS4,EME1,MMS4,

    Tissue Specificity: Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas,and which correlates with tumor progression and invasiveness or risk of progression. .

    Background: Crossover junction endonuclease EME1 is an enzyme that in humans is encoded by the EME1 gene. It is mapped to 17q21.33. This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. Also, this protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.

    Molekulargewicht

    62 kDa

    Gen-ID

    146956
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