Vimentin Antikörper (C-Term)

Produktnummer ABIN3042344

Der Kaninchen Polyklonal Anti-Vimentin-Antikörper wurde für WB, IHC und IF validiert. Er ist geeignet, Vimentin in Proben von Human, Maus und Ratte zu detektieren. Es sind 86+ Publikationen verfügbar.

Kurzübersicht für Vimentin Antikörper (C-Term) (ABIN3042344)

Target: Vimentin (VIM)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Vimentin Antikörper

Bindungsspezifität: AA 435-466, C-Term

Verwendungszweck: Anti-Vimentin Antibody Picoband®

Sequenz: DTHSKRTLLI KTVETRDGQV INETSQHHDD LE

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Vimentin Antibody Picoband® (ABIN3042344). Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Mouse, Rat
1. Besarani D, et al. Role of anti-vimentin antibodies in renal transplantation. Transplantation, 2014 Jul 15. 2. Kinloch AJ, et al. Vimentin is a dominant target of in situ humoral immunity in human lupus tubulointerstitial nephritis. Arthritis Rheumatol, 2014 Dec. 3. Shirahata A, et al. Serum vimentin methylation as a potential marker for colorectal cancer. Anticancer Res, 2014 Aug.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Vimentin Antikörper (ABIN3042344)

Li, Zang, He, Chen, Yu, Pei, Hou, An, Yang, Zhang, Liu: "Expression of pannexin 1 and 2 in cortical lesions from intractable epilepsy patients with focal cortical dysplasia." in: Oncotarget, Vol. 8, Issue 4, pp. 6883-6895, (2018) (PubMed).

Xu, Xiao, Luo, Chen, Zhang, Tao, Jiang, Chen, Shen: "Inhibitory effects of oxymatrine on TGF‑β1‑induced proliferation and abnormal differentiation in rat cardiac fibroblasts via the p38MAPK and ERK1/2 signaling pathways." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 5354-5362, (2018) (PubMed).

Bu, Zhang, Wang, Lai: "Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest." in: International journal of oncology, Vol. 51, Issue 5, pp. 1405-1414, (2018) (PubMed).

Gu, Fang, Kang, Hu, Yu, Li, Cheng, Gao: "Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 9257967, (2018) (PubMed).

Zhang, Bu, Sun, Xu, Yao, He, Lai: "Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 270, (2018) (PubMed).

Huang, Zheng, Pan, Wu, Su, Li, Wu, Sun: "Oral exposure of low-dose bisphenol A promotes proliferation of dorsolateral prostate and induces epithelial-mesenchymal transition in aged rats." in: Scientific reports, Vol. 8, Issue 1, pp. 490, (2018) (PubMed).

Ling, Zhou, Zhou, Dai, Wu, Mi, Mao, Chen: "Lin28/microRNA-let-7a promotes metastasis under circumstances of hyperactive Wnt signaling in esophageal squamous cell carcinoma." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5265-5271, (2018) (PubMed).

Jia, Jiang, Liu, Wang, Zhu, Zhu, Liu, Zhong, Xie, Huang, Jia, Li, Liu, Zuo, Cheng, Dai, Ren: "Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells." in: International journal of oncology, Vol. 52, Issue 6, pp. 1787-1800, (2018) (PubMed).

Zhang, Nguyen, Shi, Burrell, Cullen, Le: "3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration." in: Scientific reports, Vol. 8, Issue 1, pp. 6634, (2018) (PubMed).

Wang, Xu, Fu, Chen, Yang: "The Antihelminthic Niclosamide Inhibits Cancer Stemness, Extracellular Matrix Remodeling, and Metastasis through Dysregulation of the Nuclear β-catenin/c-Myc axis in OSCC." in: Scientific reports, Vol. 8, Issue 1, pp. 12776, (2018) (PubMed).

Quan, Du, Hou, Wang, Zhang: "Utilization of E-cadherin by monocytes from tumour cells plays key roles in the progression of bone invasion by oral squamous cell carcinoma." in: Oncology reports, Vol. 38, Issue 2, pp. 850-858, (2017) (PubMed).

Guo, Yu, Xu, Lian, Yu, Cui, Ji, Chen, Li, Liu, Chen: "PAC1R agonist maxadilan enhances hADSC viability and neural differentiation potential." in: Journal of cellular and molecular medicine, Vol. 20, Issue 5, pp. 874-90, (2017) (PubMed).

Deng, Li, Lin, Huang, Zeng, Wang, Li, Jin, Zhang, Li, Chen, Wang, Huang, Shao, Xu, Mao: "P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3." in: Theranostics, Vol. 6, Issue 2, pp. 204-18, (2017) (PubMed).

Zheng, Zou, Shen, Xu, Wang, Fu, Fan: "Opposite Effects of Coinjection and Distant Injection of Mesenchymal Stem Cells on Breast Tumor Cell Growth." in: Stem cells translational medicine, Vol. 5, Issue 9, pp. 1216-28, (2017) (PubMed).

Yu, Liu, Liang, Ochs, Chen, Chen, Du, Tang, Huang, Du, Jin: "Sulfur Dioxide Protects Against Collagen Accumulation in Pulmonary Artery in Association With Downregulation of the Transforming Growth Factor β1/Smad Pathway in Pulmonary Hypertensive Rats." in: Journal of the American Heart Association, Vol. 5, Issue 10, (2017) (PubMed).

Wang, Tu, Zhang, Xu, Qian: "Stellate Cell Activation and Imbalanced Expression of TGF-β1/TGF-β3 in Acute Autoimmune Liver Lesions Induced by ConA in Mice." in: BioMed research international, Vol. 2017, pp. 2540540, (2017) (PubMed).

Wei, Zhu, Xiang, Song, Guo, Lin, Li, Zeng: "Corticosterone suppresses IL-1β-induced mPGE2 expression through regulation of the 11β-HSD1 bioactivity of synovial fibroblasts in vitro." in: Experimental and therapeutic medicine, Vol. 13, Issue 5, pp. 2161-2168, (2017) (PubMed).

Wang, An, Yu, Zhang, Tang, Zhang, Li: "MiR-29b/TET1/ZEB2 signaling axis regulates metastatic properties and epithelial-mesenchymal transition in breast cancer cells." in: Oncotarget, Vol. 8, Issue 60, pp. 102119-102133, (2017) (PubMed).

Wu, Wang, Ji, Ou, Xia, Zhang, Zhao: "C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells." in: American journal of translational research, Vol. 9, Issue 12, pp. 5708-5718, (2017) (PubMed).

Deng, Guo, Liu, Zeng, Ning, Pei, Li, Chen, Guo, Li, Han, Xu: "Blocking protein phosphatase 2A signaling prevents endothelial-to-mesenchymal transition and renal fibrosis: a peptide-based drug therapy." in: Scientific reports, Vol. 6, pp. 19821, (2016) (PubMed).

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: VIM

Hintergrund:

Synonyms: Vimentin,VIM,

Tissue Specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. .

Background: VIM (vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Sequence Similarities: Belongs to the intermediate filament family.

Molekulargewicht: 54 kDa

Gen-ID: 7431

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose