ZEB2 Antikörper (AA 1-200)

Produktnummer ABIN3042325

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch ZEB2 in WB, IHC, ICC, FACS und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ZEB2 Antikörper (AA 1-200) (ABIN3042325)

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZEB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ZEB2 Antikörper

Bindungsspezifität: AA 1-200

Verwendungszweck: Anti-Smad Interacting Protein 1/ZEB2 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Smad Interacting Protein 1/ZEB2 Antibody (ABIN3042325). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ZEB2

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Andere Bezeichnung: ZEB2

Hintergrund:

Synonyms: Zinc finger E-box-binding homeobox 2,Smad-interacting protein 1,SMADIP1,Zinc finger homeobox protein 1b,ZEB2,KIAA0569, SIP1, ZFHX1B, ZFX1B,HRIHFB2411,

Tissue Specificity: Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. .

Background: ZEB2 (Zinc finger E-box-binding homeobox2), also known as SIP1 or ZINC FINGER HOMEOBOX 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation (NURD) complex. By radiation hybrid analysis, this gene is mapped to 2q22. It has been found that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines, and the expression of SNAI1 in epithelial cells can trigger an epithelial-mesenchyme transition.

Sequence Similarities: Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Molekulargewicht: 200 kDa

Gen-ID: 9839

UniProt: O60315

Pathways: Tube Formation