MHC Class I H-2Ld Antikörper (Biotin)

Produktnummer ABIN303513

Produktdetails

Target: MHC Class I H-2Ld

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Flow Cytometry (FACS)

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Protein G Chromatography.

Klon: 30-5-7S

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (See Protocol below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: MHC Class I H-2Ld

Hintergrund: The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.Synonyms: H-2 class I histocompatibility antigen, H2-L, L-D alpha chain

Gen-ID: 10090

UniProt: P01897