PDIA3 Antikörper (AA 172-188)

Produktnummer ABIN3032368

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PDIA3 in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PDIA3 Antikörper (AA 172-188) (ABIN3032368)

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDIA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PDIA3 Antikörper

Bindungsspezifität: AA 172-188

Aufreinigung: Antigen affinity

Immunogen: Amino acids 172-188 (LKAASNLRDNYRFAHTN) were used as the immunogen for this ERp57 antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the ERp57 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the ERp57 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Andere Bezeichnung: ERp57

Hintergrund: PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, ERp57 or ER60, is an isomerase enzyme, mapped on 15q15.3. The PDIA3 protein has protein disulfide isomerase activity. It is also part of the major histocompatibility complex(MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Koivunen et al. showed that PDIA3/ERp57 could not substitute for the beta subunit of PDI. When coexpressed with alpha prolyl 4-hydroxylase, it did not form prolyl 4-hydroxylase tetramers, nor did it have prolyl 4-hydroxylase activity. Hirano et al. expressed human ERp57 and found that the protein had a thiol-dependent reductase activity. They showed that the expression level increased after oncogenic transformation of normal rat kidney cells and NIH 3T3 cells.

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis