Peroxiredoxin 3 Antikörper (AA 110-256)

Produktnummer ABIN3032189

Der Kaninchen Polyklonal Anti-Peroxiredoxin 3-Antikörper wurde für WB, ICC und IHC (p) validiert. Er ist geeignet, Peroxiredoxin 3 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für Peroxiredoxin 3 Antikörper (AA 110-256) (ABIN3032189)

Target: Peroxiredoxin 3 (PRDX3)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Peroxiredoxin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Peroxiredoxin 3 Antikörper

Bindungsspezifität: AA 110-256

Aufreinigung: Antigen affinity

Immunogen: Human partial recombinant protein (AA 110-256) was used as the immunogen for this PRDX3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the PRDX3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,ICC: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the PRDX3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu Peroxiredoxin 3

Target: Peroxiredoxin 3 (PRDX3)

Andere Bezeichnung: PRDX3 (Peroxiredoxin 3)

Hintergrund: Peroxiredoxin 3, also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human protein shares 86 % amino acid sequence similarity with mouse, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of Peroxiredoxin 3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that Peroxiredoxin 3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.

Gen-ID: 10935

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process