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Myosin Binding Subunit Antikörper (N-Term)

Dieses Anti-Myosin Binding Subunit-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Myosin Binding Subunit in WB und FACS. Geeignet für Human, Maus und Ratte.
Produktnummer ABIN3031917

Kurzübersicht für Myosin Binding Subunit Antikörper (N-Term) (ABIN3031917)

Target

Myosin Binding Subunit (MBS)

Reaktivität

Human, Maus, Ratte

Wirt

  • 1
Kaninchen

Klonalität

  • 1
Polyklonal

Konjugat

  • 1
Dieser Myosin Binding Subunit Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Flow Cytometry (FACS)
  • Bindungsspezifität

    N-Term

    Aufreinigung

    Antigen affinity

    Immunogen

    An amino acid sequence from the N-terminus of human Myosin Phosphatase (MKMADAKQKRNEQLKRW) was used as the immunogen for this Myosin Phosphatase antibody (100% homologous in human, mouse and rat).

    Isotyp

    IgG
  • Applikationshinweise

    The stated application concentrations are suggested starting amounts. Titration of the Myosin Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL, FACS: 1-3 μg/10^6 cells

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Buffer

    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

    Lagerung

    -20 °C

    Informationen zur Lagerung

    After reconstitution, the Myosin Phosphatase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • Target

    Myosin Binding Subunit (MBS)

    Andere Bezeichnung

    Myosin Phosphatase

    Hintergrund

    PPP1R12A, also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.

    UniProt

    O14974
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