MYL9 Antikörper (AA 12-27)

Produktnummer ABIN303105

Dieses Anti-MYL9-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MYL9 in WB, IP, IHC (p) und EIA. Geeignet für Human, Maus, Ratte, Avian und Säugetier.

Kurzübersicht für MYL9 Antikörper (AA 12-27) (ABIN303105)

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Reaktivität: Human, Maus, Ratte, Avian, Säugetier

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYL9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-MYL9 Antikörper

Bindungsspezifität: AA 12-27

Spezifität: Amino acids 12-27 of Human Myosin light chain protein.

Kreuzreaktivität (Details): Species reactivity (expected):Avian, Mammal, Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated.

Anwendungsinformationen

Applikationshinweise: ELISA (1/5000-1/20000). Western Blot (1/500-1/2000). Immunoprecipitation. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by Alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.71 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preserevative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu MYL9

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Andere Bezeichnung: MYL9

Hintergrund: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/ cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, Mouse and Rat have almost identical sequences.Synonyms: LC20, MLC-2C, MLC2, MRLC1, MYRL2, Myosin RLC, Myosin regulatory light chain 2, Myosin regulatory light chain MRLC1, Myosin regulatory light polypeptide 9, smooth muscle

Gen-ID: 10398

NCBI Accession: NP_006088

UniProt: P24844