CRTC1 Antikörper

Produktnummer ABIN303081

Dieses Anti-CRTC1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CRTC1 in WB, IF, IHC (p) und EIA. Geeignet für Human, Maus, Ratte, Rind (Kuh) und Zebrafisch (Danio rerio).

Kurzübersicht für CRTC1 Antikörper (ABIN303081)

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CRTC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-CRTC1 Antikörper

Spezifität: This antibody recognizes amino acids 19-34 of Human MECT1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Zebrafish, Bovine.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Synthetic peptide from Human CRTC1 / MECT1.

Anwendungsinformationen

Applikationshinweise: Immunofluorescence. Immunohistochemistry on paraffin sections: 1: 500. Western Blot: 1: 500 - 1: 1000. ELISA: 1: 10000 - 1: 40000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu CRTC1

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Andere Bezeichnung: CRTC1 / MECT1

Hintergrund: MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1, Transducer of regulated cAMP response element-binding protein 1, TORC1, and Transducer of CREB protein 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11,19) (q21,p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein.Synonyms: CREB-regulated transcription coactivator 1, KIAA0616, Mucoepidermoid carcinoma translocated protein 1, TORC1, Transducer of regulated cAMP response element-binding protein 1, WAMTP1

Gen-ID: 23373

NCBI Accession: NP_001091952

UniProt: Q6UUV9