DCI Antikörper (C-Term)

Produktnummer ABIN3030698

Der Kaninchen Polyklonal anti-DCI Antikörper wird verwendet zum Nachweis von DCI in Proben von Human, Maus und Ratte. Er wurde validiert für WB, FACS und IHC (p).

Kurzübersicht für DCI Antikörper (C-Term) (ABIN3030698)

Target: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DCI Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DCI Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: DCI Antibody / Dodecenoyl-CoA Delta Isomerase / ECI1

Sequenz: ADVQNFVSFI SKDSIQKSL

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human DCI (ADVQNFVSFISKDSIQKSL) was used as the immunogen for this DCI antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the DCI antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the DCI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu DCI

Target: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Andere Bezeichnung: DCI

Hintergrund: DCI Antibody targets Dodecenoyl-CoA delta isomerase, encoded by the ECI1 gene. Dodecenoyl-CoA delta isomerase is a mitochondrial enzyme that plays a critical role in the beta-oxidation of unsaturated fatty acids. This enzyme catalyzes the isomerization of cis- or trans-unsaturated enoyl-CoA intermediates, enabling their continued processing through the mitochondrial fatty acid degradation pathway. As such, Dodecenoyl-CoA delta isomerase serves as an essential auxiliary enzyme that ensures efficient energy production from dietary and stored lipids.

Functionally, Dodecenoyl-CoA delta isomerase acts downstream of acyl-CoA dehydrogenases and enoyl-CoA hydratases, converting specific unsaturated fatty acyl-CoA substrates into configurations compatible with the core beta-oxidation machinery. Without this isomerization step, certain unsaturated fatty acids would accumulate as metabolic dead ends, reducing overall oxidative capacity. A DCI Antibody is therefore useful for studying mitochondrial lipid metabolism, energy homeostasis, and the regulation of fatty acid utilization under physiological and stress conditions.

Expression of Dodecenoyl-CoA delta isomerase is observed in many metabolically active tissues, including liver, muscle, heart, and kidney, reflecting the widespread requirement for fatty acid oxidation. Subcellular localization is predominantly mitochondrial, consistent with its enzymatic role within the beta-oxidation pathway. The protein contains a conserved catalytic domain characteristic of enoyl-CoA isomerases, supporting substrate binding and stereochemical conversion of fatty acyl-CoA intermediates. Studies often examine ECI1 localization and abundance to better understand mitochondrial function and metabolic flexibility.

From a disease relevance perspective, alterations in mitochondrial fatty acid oxidation pathways have been linked to metabolic disorders, insulin resistance, and inherited defects of lipid metabolism. While ECI1 is not among the most frequently mutated beta-oxidation enzymes, changes in its expression or activity may contribute to broader mitochondrial dysfunction and impaired lipid handling. Dodecenoyl-CoA delta isomerase has also been examined in the context of cancer metabolism, where reprogramming of fatty acid oxidation can support tumor growth and survival under nutrient-limited conditions.

At the molecular level, Dodecenoyl-CoA delta isomerase functions as a soluble mitochondrial matrix protein, interacting indirectly with other beta-oxidation enzymes through coordinated metabolic flux rather than stable protein complexes. Antibody-based detection of Dodecenoyl-CoA delta isomerase supports research into mitochondrial biology, lipid metabolism, and metabolic disease mechanisms. NSJ Bioreagents provides reagents intended for research use to support investigations involving ECI1 expression and mitochondrial fatty acid oxidation pathways.

UniProt: P42126