AQP2 Antikörper (AA 245-260)

Produktnummer ABIN3030003

Dieses Anti-AQP2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von AQP2 in WB, IHC (p) und IHC (fro). Geeignet für Human, Ratte und Maus.

Kurzübersicht für AQP2 Antikörper (AA 245-260) (ABIN3030003)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-AQP2 Antikörper

Bindungsspezifität: AA 245-260

Aufreinigung: Antigen affinity

Immunogen: Amino acids 245-260 (DWEEREVRRRQSVELH) were used as the immunogen for this AQP2 antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the AQP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the AQP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Andere Bezeichnung: AQP2 Aquaporin 2

Hintergrund: Aquaporin 2 also called Aquaporin-CD, is found in the apical cell membranes of the kidneys collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. Investigators suggest that a defect in the gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aquaporin 2, trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide(NO). The NO donors sodium nitroprusside(SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin(VP), stimulating relocation of Aquaporin 2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. Expression in kidney connecting tubules is sufficient for survival and that expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the protein prevented phosphorylation at S256 and the subsequent accumulation of Aquaporin 2 on the apical membrane of the collecting duct principal cells.

UniProt: P41181

Pathways: Response to Water Deprivation