IDO Antikörper (N-Term)

Produktnummer ABIN3029730

Dieses Anti-IDO-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IDO in WB. Geeignet für Human.

Kurzübersicht für IDO Antikörper (N-Term) (ABIN3029730)

Target: IDO (Indoleamine 2,3-Dioxygenase (IDO))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IDO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-IDO Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human Indoleamine 2, 3-dioxygenase (MAHAMENSWTISKEYHIDEE) was used as the immunogen for this IDO antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the IDO antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the IDO antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu IDO

Target: IDO (Indoleamine 2,3-Dioxygenase (IDO))

Andere Bezeichnung: IDO Indoleamine 2, 3-Dioxygenase

Hintergrund: Indoleamine 2,3-dioxygenase, also called INDO or IDO, is an immunomodulatory enzyme produced by some alternatively activated macrophages and other immunoregulatory cells. This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. By fluorescence in situ hybridization, the assignment is narrowed to chromosome 8p12-p11. IDO-Interferon-gamma interaction has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase. During inflammation, IDO is upregulated in dendritic cells and phagocytes by proinflammatory stimuli, most notably IFNG, and the enzyme then uses superoxide as a 'cofactor' for oxidative cleavage of the indole ring of tryptophan, yielding an intermediate that deformylates to L-kynurenine.

UniProt: P14902