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Secretory Component Glycoprotein Antikörper

Der Maus Monoklonal Anti-Secretory Component Glycoprotein-Antikörper wurde für IHC (p), IF und FACS validiert. Er ist geeignet, Secretory Component Glycoprotein in Proben von Human und Ratte zu detektieren.
Produktnummer ABIN3026937

Kurzübersicht für Secretory Component Glycoprotein Antikörper (ABIN3026937)

Target

Secretory Component Glycoprotein

Reaktivität

Human, Ratte

Wirt

  • 3
Maus

Klonalität

  • 3
Monoklonal

Konjugat

  • 3
Dieser Secretory Component Glycoprotein Antikörper ist unkonjugiert

Applikation

  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon

SPM217
  • Produktmerkmale

    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

    Aufreinigung

    Protein G affinity chromatography

    Immunogen

    Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Konservierungsmittel

    Azide free

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store the Secretory Component antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    Secretory Component Glycoprotein

    Hintergrund

    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
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