Epithelial Marker Antigen Antikörper

Produktnummer ABIN3026793

Der Maus Monoklonal anti-Epithelial Marker Antigen Antikörper wird verwendet zum Nachweis von Epithelial Marker Antigen in Proben von Human. Er wurde validiert für FACS, IF und IHC (p).

Kurzübersicht für Epithelial Marker Antigen Antikörper (ABIN3026793)

Target: Epithelial Marker Antigen

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MUC1-967

Produktdetails

Aufreinigung: Protein G affinity chromatography

Reinheit: Protein G affinity chromatography

Immunogen: Human milk fat globule membranes were used as the immunogen for this Epithelial Marker Antigen antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Epithelial Marker Antigen antibody to be titered up or down for optimal performance.

1. FFPE staining REQUIRES boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 10-20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Epithelial marker antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Antigendetails

Target: Epithelial Marker Antigen

Hintergrund: This Epithelial Membrane Antigen / EMA antibody, also called MUC1 and Mucin-1, recognizes the full-length protein in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this mAb is APDTR in the VNTR region. It reacts with the core peptide of the EMA protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200  kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the mAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This EMA antibody reacts with both normal and malignant epithelia of various tissues including breast and colon.

Gen-ID: 4582