UCHL1 Antikörper

Produktnummer ABIN3026779

Der Maus Monoklonal anti-UCHL1 Antikörper (Klon 31A3) (ABIN3026779) detektiert spezifisch UCHL1 in WB, IF, FACS und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für UCHL1 Antikörper (ABIN3026779)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser UCHL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: 31A3

Produktdetails anti-UCHL1 Antikörper

Verwendungszweck: PGP9.5 Antibody / UchL1 (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: Native protein from brain was used as the immunogen for this PGP9.5 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PGP9.5 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Andere Bezeichnung: PGP9.5

Hintergrund: This antibody specifically reacts with a protein of 20-30 kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. PGP9.5 antibody has been used to show the protein to be a useful prognostic indicator of colorectal, pancreatic and gall bladder cancer. A mutation in the gene is believed to cause a form of Parkinson's disease.

Pathways: Feeding Behaviour