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Granulocyte Marker Antikörper

Reaktivität: Human, Makake FACS, IF, IHC (p) Wirt: Maus Monoclonal BM-2 unconjugated
Produktnummer ABIN3026709
  • Target
    Granulocyte Marker
    Reaktivität
    Human, Makake
    Wirt
    • 12
    Maus
    Klonalität
    • 12
    Monoklonal
    Konjugat
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Unkonjugiert
    Applikation
    • 11
    • 11
    • 9
    • 9
    • 9
    • 3
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Produktmerkmale
    This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas.
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this Granulocyte Marker antibody.
    Klon
    BM-2
    Isotyp
    IgG1
  • Applikationshinweise
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Granulocyte Marker antibody to be titered up or down for optimal performance.

    1. No special pretreatment is required for staining of formalin/paraffin tissues.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Konservierungsmittel
    Azide free
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the Granulocyte Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Granulocyte Marker
    Hintergrund
    This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas.
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