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Actin, Muscle Specific Antikörper

Dieses Anti-Actin, Muscle Specific-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Actin, Muscle Specific in FACS, IF und IHC (p). Geeignet für Human, Kaninchen und Ratte.
Produktnummer ABIN3026680

Kurzübersicht für Actin, Muscle Specific Antikörper (ABIN3026680)

Target

Actin, Muscle Specific

Reaktivität

  • 30
  • 29
  • 29
  • 10
  • 10
  • 10
  • 10
Human, Kaninchen, Ratte

Wirt

  • 30
Maus

Klonalität

  • 30
Monoklonal

Konjugat

  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
Dieser Actin, Muscle Specific Antikörper ist unkonjugiert

Applikation

  • 26
  • 26
  • 18
  • 11
  • 5
  • 3
  • 1
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

SPM160
  • Produktmerkmale

    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

    Aufreinigung

    Protein G affinity chromatography

    Immunogen

    Human myocardium extract was used as the immunogen for this Muscle Specific Actin antibody.

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    The optimal dilution of the Muscle Specific Actin antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Konservierungsmittel

    Azide free

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store the Muscle Specific Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    Actin, Muscle Specific

    Andere Bezeichnung

    Muscle Specific Actin

    Hintergrund

    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
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