HCG beta Antikörper

Produktnummer ABIN3026412

Der Maus Monoklonal Anti-HCG beta-Antikörper wurde für ELISA und IHC (p) validiert. Er ist geeignet, HCG beta in Proben von Human zu detektieren.

Kurzübersicht für HCG beta Antikörper (ABIN3026412)

Target: HCG beta

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HCG beta Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: HCGb-54

Produktdetails anti-HCG beta Antikörper

Produktmerkmale: This antibody reacts with a protein of 22 kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for this HCG-beta antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.

1. Clone HCGb/54 (recommended detect) will pair with clones < a href=../tds/hcg-beta-antibody-hcgb211-v2374>HCGb/211 (recommended capture) and < a href=../tds/hcg-beta-antibody-hcgb459-v2373>HCGb/459 by ELISA.
2. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: use BSA-free format (1),IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (2),Prediluted format : incubate for 30 min at RT (3)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the HCG-beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu HCG beta

Target: HCG beta

Andere Bezeichnung: HCG-beta

Hintergrund: This antibody reacts with a protein of 22  kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Gen-ID: 1082