CD99 Antikörper

Produktnummer ABIN3025995

Der Maus Monoklonal Anti-CD99-Antikörper wurde für FACS, IF und IHC (p) validiert. Er ist geeignet, CD99 in Proben von Human zu detektieren.

Kurzübersicht für CD99 Antikörper (ABIN3025995)

Target: CD99

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD99 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 12E7-MIC2-877

Produktdetails anti-CD99 Antikörper

Reinheit: Unpurified high titer supernatant

Immunogen: Human acute lymphocytic leukemia T-cells (12E7) and recombinant human protein (MIC2/877) was used as the immunogen for the CD99 antibody cocktail.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CD99 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 5-10 μL/million cells in 0.1ml,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Bioreactor concentrate with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store the CD99 antibody c°Cktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD99

Target: CD99

Andere Bezeichnung: CD99

Hintergrund: Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.