Cytokeratin 19 Antikörper

Produktnummer ABIN3025850

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytokeratin 19 in IF, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Cytokeratin 19 Antikörper (ABIN3025850)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 19 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: KRT19-799-KRT19-800

Produktdetails anti-Cytokeratin 19 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant full-length human KRT19 protein was used as the immunogen for the Cytokeratin 19 antibody cocktail.

Isotyp: IgG1, IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Cytokeratin 19 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cytokeratin 19 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung: Cytokeratin 19

Hintergrund: Recognizes a protein of 40  kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.