L1 Antikörper
Kurzübersicht für L1 Antikörper (ABIN3025473)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Produktmerkmale
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Reacts with a protein of 57 kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2. [UniProt]
The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33, very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
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Aufreinigung
- Protein G affinity chromatography
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Immunogen
- Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody.
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Isotyp
- IgG2a kappa
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anti-L1 antibody
Reaktivität: Human Papillomavirus 16 (HPV-16) ELISA, IF, IHC (p), WB Wirt: Maus Monoclonal CamVir-1 unconjugated
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Applikationshinweise
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Optimal dilution of the L1 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL,ELISA: assay dependent,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 0.2 mg/mL
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Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store the L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- L1
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Hintergrund
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Reacts with a protein of 57 kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2. [UniProt]
The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33, very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
Target
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