CD43 Antikörper

Produktnummer ABIN3025352

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD43 in FACS, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD43 Antikörper (ABIN3025352)

Target: CD43 (SPN) (Sialophorin (SPN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: Bra7G

Produktdetails anti-CD43 Antikörper

Aufreinigung: PEG precipitation

Immunogen: Immature pluripotent human leukemia K562 cells were used as the immunogen for the CD43 antibody.

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CD43 antibody should be determined by the researcher.

1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at room temp
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: CD43

Hintergrund: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt]

Pathways: Regulation of Leukocyte Mediated Immunity