Myogenin Antikörper (AA 138-158)

Produktnummer ABIN3025317

Der Maus Monoklonal Anti-Myogenin-Antikörper wurde für WB, ELISA, IF, FACS, IP und IHC (p) validiert. Er ist geeignet, Myogenin in Proben von Human, Maus, Ratte, Schwein, Cat und Hund zu detektieren.

Kurzübersicht für Myogenin Antikörper (AA 138-158) (ABIN3025317)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reaktivität: Human, Maus, Ratte, Schwein, Cat, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Myogenin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: F5D

Produktdetails anti-Myogenin Antikörper

Bindungsspezifität: AA 138-158

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the Myogenin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order BSA-free format,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunoprecipitation: 0.5-1 μg/500 μg protein lysate,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Myogenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Andere Bezeichnung: Myogenin

Hintergrund: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development