MLANA Antikörper

Produktnummer ABIN3025225

Dieses Anti-MLANA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MLANA in IF, FACS und IHC (p). Geeignet für Human und Pferd.

Kurzübersicht für MLANA Antikörper (ABIN3025225)

Target: MLANA (Melan A (MLANA))

Reaktivität: Human, Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MLANA Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM342

Produktdetails anti-MLANA Antikörper

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for this anti-Melan-A antibody.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-Melan-A antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-Melan-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu MLANA

Target: MLANA (Melan A (MLANA))

Andere Bezeichnung: Melan-A

Hintergrund: Melan-A/MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1/Melan-A is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.